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Figure 3 | Journal of Biomedical Science

Figure 3

From: Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity

Figure 3

Kinetic analysis of human EndoG. (A) Electrophoresis analysis. EndoG (0.05 pmol) was mixed with various amounts of supercoiled pUC18 dsDNA and incubated at 37°C for 3 min. The resulting products were then analyzed by 1.2% agarose gels. Arrowheads denote the different topological forms of pUC18 plasmids. OC: open circular; L: linear; SC: supercoiled. (B) Initial velocity of DNA cleavage as a function of substrate concentration. Steady-state kinetic parameters were determined by curve fitting of these values to the Michaelis-Menten equation.

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Journal of Biomedical Science

ISSN: 1423-0127