Figure 3From: Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificityKinetic analysis of human EndoG. (A) Electrophoresis analysis. EndoG (0.05 pmol) was mixed with various amounts of supercoiled pUC18 dsDNA and incubated at 37°C for 3 min. The resulting products were then analyzed by 1.2% agarose gels. Arrowheads denote the different topological forms of pUC18 plasmids. OC: open circular; L: linear; SC: supercoiled. (B) Initial velocity of DNA cleavage as a function of substrate concentration. Steady-state kinetic parameters were determined by curve fitting of these values to the Michaelis-Menten equation.Back to article page