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Table 1 DNA oligonucleotides for the constructions of human EndoG mutants.

From: Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity

Primer name

Primer sequencea

R110A

5'-GCACTCTCGAGC GTCGCCGTCGCCGCGGAGACG-3'

R139A

5'-CAGGTGGCCGGC GTCGAAGCCACTGCCGCGG-3'

H141A

5'-GGCCAGGGC CCCGCGGTCGAAGCCACTGCC-3'

H141D

5'-GGCCAGATC TCCGCGGTCGAAGCCACTGCCGC-3'

N163A

5'-GGGGCGCTACAGC GCTCAGGTAGAACGTGTCGTCC-3'

N163K

5'-CACCTGGGGCGCGACCTT GCTCAGGTAGAAC-3'

N172A

5'-CATTCTGCGC AAGGTGGGGCACCTGGGGCGCG-3'

R184A

5'-TCAAGCTTGC GCTATATTTCTCCAGGTTGTTCC-3'

H228A

5'-TTGAAGAATGC TGTGGGCACTGCCACGTGG-3'

H228D

5'-GAAGAAGTC TGTAGGCACTGCCACGTGGTTCTTGCC-3'

N251A

5'-AGGTGCTGC AGGCATCACGTAGGTGCGGAGC-3'

E271A

5'-CGAAGCCCGAGC AATGCTCTCGATGGGCACCAGG-3'

R272A

5'-CAGCCCTGAGGCCGC CTCAATGCTCTCGATGGGCACC-3'

  1. a Bold sequences indicate codon changes
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Journal of Biomedical Science

ISSN: 1423-0127