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Table 1 DNA oligonucleotides for the constructions of human EndoG mutants.

From: Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity

Primer name Primer sequencea
R110A 5'-GCACTCTCGAGC GTCGCCGTCGCCGCGGAGACG-3'
R139A 5'-CAGGTGGCCGGC GTCGAAGCCACTGCCGCGG-3'
H141A 5'-GGCCAGGGC CCCGCGGTCGAAGCCACTGCC-3'
H141D 5'-GGCCAGATC TCCGCGGTCGAAGCCACTGCCGC-3'
N163A 5'-GGGGCGCTACAGC GCTCAGGTAGAACGTGTCGTCC-3'
N163K 5'-CACCTGGGGCGCGACCTT GCTCAGGTAGAAC-3'
N172A 5'-CATTCTGCGC AAGGTGGGGCACCTGGGGCGCG-3'
R184A 5'-TCAAGCTTGC GCTATATTTCTCCAGGTTGTTCC-3'
H228A 5'-TTGAAGAATGC TGTGGGCACTGCCACGTGG-3'
H228D 5'-GAAGAAGTC TGTAGGCACTGCCACGTGGTTCTTGCC-3'
N251A 5'-AGGTGCTGC AGGCATCACGTAGGTGCGGAGC-3'
E271A 5'-CGAAGCCCGAGC AATGCTCTCGATGGGCACCAGG-3'
R272A 5'-CAGCCCTGAGGCCGC CTCAATGCTCTCGATGGGCACC-3'
  1. a Bold sequences indicate codon changes