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Figure 3 | Journal of Biomedical Science

Figure 3

From: Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide-induced inflammation in vitro and in vivo

Figure 3

Effects of ASEA on IL-6 mRNA expression and NF-κB activation in LPS-stimulated RAW264.7 cells. (A) Cells pre-treated with 0 (control), 10, 20 or 50 μg/ml ASEA for 1 hr were stimulated with LPS for IL-6 mRNA expression by real-time PCR. (B) Cells transfected with the 3x-κB-tk-luciferase and pRL-tk-luciferse plasmids were stimulated with LPS and IFN-γ in the absence (0) or the presence of 2, 10, and 50 μg/ml ASEA for the reporter assay. (C) Nuclear extracts from unstimulated (spontaneous) or LPS-stimulated cells pre-treated with 0, 20, or 50 μg/ml ASEA were for DNA-binding activities of the NF-κB subunit, p65. Bar values are mean ± SD in three independent experiments in these assays. A significant difference from the control was indicated as *p < 0.05 or **p < 0.01 by Student's t-test.

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