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Table 1 Effects of ASEA on pro-inflammatory cytokines production from LPS-stimulated RAW264.7 cells and peritoneal macrophages.

From: Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide-induced inflammation in vitro and in vivo

Treatment RAW 264.7, ng/mla Macrophages, ng/mlb
  IL-1β IL-6 TNF-α IL-1β IL-6 TNF-α
Control (0) 0.16 ± 0.02 36.3 ± 1.61 12.3 ± 2.63 1.22 ± 0.22 36.3 ± 5.75 1.67 ± 0.21
ASEA 2 μg/ml 0.19 ± 0.01 32.1 ± 0.62 10.9 ± 4.01 1.13 ± 0.24 41.6 ± 9.12 1.77 ± 0.25
ASEA 10 μg/ml 0.17 ± 0.02 20.8 ± 2.88* 14.6 ± 2.71 1.06 ± 0.32 34.6 ± 6.68 1.44 ± 0.29
ASEA 50 μg/ml 0.06 ± 0.01* 17.4 ± 5.81** 14.3 ± 1.72 0.76 ± 0.24* 16.4 ± 5.55* 1.41 ± 0.15
PDTC100 μM 0.14 ± 0.03 15.9 ± 3.24** 1.58 ± 0.39**   N.D.c  
  1. Values are expressed as means ± SD of three independent experiments with triplicates each and statistically analyzed by using Student's t-test. A significant difference from the control was indicated *p < 0.05.
  2. aRAW264.7 cells were stimulated 100 ng/ml LPS for 48-hr incubation.
  3. bMurine peritoneal macrophages were stimulated with 10 μg/ml LPS for 48-hr incubation.
  4. cN.D. means no detection in cytokine profile of peritoneal macrophages.