Skip to main content

Table 1 Effects of ASEA on pro-inflammatory cytokines production from LPS-stimulated RAW264.7 cells and peritoneal macrophages.

From: Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide-induced inflammation in vitro and in vivo

Treatment

RAW 264.7, ng/mla

Macrophages, ng/mlb

 

IL-1β

IL-6

TNF-α

IL-1β

IL-6

TNF-α

Control (0)

0.16 ± 0.02

36.3 ± 1.61

12.3 ± 2.63

1.22 ± 0.22

36.3 ± 5.75

1.67 ± 0.21

ASEA 2 μg/ml

0.19 ± 0.01

32.1 ± 0.62

10.9 ± 4.01

1.13 ± 0.24

41.6 ± 9.12

1.77 ± 0.25

ASEA 10 μg/ml

0.17 ± 0.02

20.8 ± 2.88*

14.6 ± 2.71

1.06 ± 0.32

34.6 ± 6.68

1.44 ± 0.29

ASEA 50 μg/ml

0.06 ± 0.01*

17.4 ± 5.81**

14.3 ± 1.72

0.76 ± 0.24*

16.4 ± 5.55*

1.41 ± 0.15

PDTC100 μM

0.14 ± 0.03

15.9 ± 3.24**

1.58 ± 0.39**

 

N.D.c

 
  1. Values are expressed as means ± SD of three independent experiments with triplicates each and statistically analyzed by using Student's t-test. A significant difference from the control was indicated *p < 0.05.
  2. aRAW264.7 cells were stimulated 100 ng/ml LPS for 48-hr incubation.
  3. bMurine peritoneal macrophages were stimulated with 10 μg/ml LPS for 48-hr incubation.
  4. cN.D. means no detection in cytokine profile of peritoneal macrophages.
Back to article page

Journal of Biomedical Science

ISSN: 1423-0127