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Table 2 The effect of ASEA supplement on the production of pro-inflammatory cytokines in serum of LPS-challenged BALB/c mice.

From: Ethyl acetate extracts of alfalfa (Medicago sativa L.) sprouts inhibit lipopolysaccharide-induced inflammation in vitro and in vivo

Cytokine Time after LPS Control ASEA PDTC
   n = 15 n = 16 n = 9
   Serum cytokine levels, ng/ml
TNF-α 2 hr 7.51 ± 1.37 5.63 ± 1.01 0.87 ± 0.32 **
  9 hr 0.63 ± 0.09 0.31 ± 0.06 * 0.16 ± 0.03 *
IL-6 2 hr 286 ± 28 235 ± 15 299 ± 19
  9 hr 307 ± 43 145 ± 26 ** 49 ± 43 **
IL-1β 2 hr 0.21 ± 0.01 0.19 ± 0.01 0.18 ± 0.01 *
  9 hr 0.38 ± 0.05 0.23 ± 0.03 * 0.12 ± 0.03 **
  1. Sera at 2 hr and 9 hr after LPS injection were collected for cytokines assay. The cytokine production in serum was assayed by ELISA. Values are means ± SEM. *p < 0.05, **p < 0.01, significantly different from the control group analyzed by Student's t-test.