Purified VP0-VP1-VP3 capsid protein complexes spontaneously formed large protein aggregates. (A) Gel filtration. Purified capsid protein complexes (Fig. 1C) were reloaded onto a size exclusion column (HiLoad 16/60 Superdex, GE Healthcare), and eluted at 1.0 ml/min. The elution volume is indicated. A significant portion of VP0-VP1-VP3 capsid protein complexes formed large protein aggregates. (B) SDS-PAGE of VP0-VP1-VP3 protein complexes from indicated fractions.