Perfusion of hearts with DETA/NO protects heart mitochondria from the loss of cytochrome c after ischemia/reperfusion. (A) – mitochondrial cytochrome c, (B) – cytosolic cytochrome c. Experimental conditions were the same as described in Fig. 3, except that rat hearts after perfusion with DETA/NO (± KT 5823) were subjected to 30 min stop-flow global ischemia followed by 30 min reperfusion. * – Statistically significant effect of ischemia/reperfusion (I/R) (p < 0.01, Tukey test) if compared to control; # – statistically significant effect of DETA/NO (p < 0.05, Tukey test) if compared to I/R. ^ – statistically significant effect of KT 5823 (p < 0.05, LSD test) if compared to I/R + DETA/NO group. Means ± standard errors of 4–9 separate experiments are presented.