Cellular responses to TRX overexpressing. (A) Levels of mRNA of HO-1, c-jun and c-fos genes in Lt-eGFP- or Lt-TRX-transduced cells. The mRNA was measured by real-time PCR. Results are shown as a ratio of indicated gene to Rps29 (housekeeping gene). Results are the mean ± S.E.M. of triplicates from three independent experiments. (B) Lt-eGFP- or Lt-TRX-transduced NIT-1 cells were transiently transfected with pCMV-luciferase and pAP1-SEAP plasmids. SEAP activity in culture supernatants was determined and normalized for luciferase activity in the cell lysates. Data are shown as means ± S.E.M. of triplicates from three independent experiments.