Representative illustration of vacuole counts and S-100. The left sciatic nerve was crushed, and the four different treatments were applied. After 4 weeks, the injured nerves were retrieved and were subjected to histological assay and antibody against S-100 for determination of vacuole counts (A-D) and myelination (E-F). Quantitative analysis is depicted in (I, J). * p < 0.05, ** p < 0.01, *** p < 0.001, n = 6, bar length = 50 μm.