Determination of inflammatory cells and associated cytokines. The nerve tissues were retrieved 7 days after injury and were subjected to IHC with antibodies against CD68, neutrophils, fibrin and ELISA studies of pro-inflammatory cytokines. The representative samples of deposits of macrophages (A-D) and neutrophils (E-H) in injured nerve are shown. The quantitative analysis of macrophages and neutrophils (I), inflammatory cytokines (J), and density of fibrin aggregation (K) is depicted. * p < 0.05, *** p < 0.001. bar length = 50 μm, n = 6.