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Table 2 Quantitative real-time PCR analysis for the mRNA expressions in sham-operated (SO) and bile-duct-ligated (BDL) rats receiving armepavine (Arm), silymarin (sil) or vehicle (0.7% CMC) treatment

From: Inhibitory effects of armepavine against hepatic fibrosis in rats

Group

SO +

BDL +

BDL + sil

BDL + Arm

BDL + Arm

 

vehicle

vehicle

(50 mg/kg)

(3 mg/kg)

(10 mg/kg)

col1α2/GAPDH

1.00 ± 0.44

12.5 ± 0.2*

7.31 ± 0.11*,#

7.45 ± 0.08*,#

5.09 ± 0.23*,#

TGF-β1/GAPDH

1.00 ± 0.81

21.5 ± 0.2*

13.1 ± 0.9*,#

14.5 ± 0.8*,#

7.09 ± 1.72*,#

TIMP-1/GAPDH

1.01 ± 0.84

28.0 ± 2.6*

4.93 ± 1.88*,#

4.36 ± 1.70*,#

3.87 ± 1.59*,#

ICAM-1/GAPDH

1.00 ± 0.20

22.5 ± 0.5*

12.2 ± 0.1*,#

15.5 ± 0.2*,#

7.03 ± 0.35*,#

iNOS/GAPDH

1.00 ± 0.08

7.53 ± 0.17*

5.31 ± 0.09*,#

6.45 ± 0.08*,#

3.09 ± 0.31*,#

IL-6/GAPDH

1.00 ± 0.10

31.5 ± 0.3*

11.3 ± 0.1*,#

10.5 ± 0.1*,#

4.09 ± 0.17*,#

Metallothionein/GAPDH

1.00 ± 0.10

0.34 ± 0.06*

0.56 ± 0.08*,#

0.37 ± 0.10*

0.57 ± 0.05*,#

  1. Note: Quantitative real-time PCR analysis for the expressions of procollagen I (col 12), transforming growth factor-β1 (TGF-β1), tissue inhibitor of metalloproteinase-1 (TIMP-1), intercellular adhension molecule-I (ICAM-1), iNOS, interleukin (IL)-6 and metallothionein genes in sham-operated rats and BDL rats receiving saline, Arm (3 and 10 mg/kg) or silymarin (50 mg/kg). Data are expressed as the mean ± SEM. The number of rats in each group is 6. *p < 0.05 vs. sham operated group; #p < 0.05 vs. BDL group.