Figure 7
A) Geldanamycin has no effect on IHC survival during the gentamicin-induced ototoxicity. Number of IHCs per 100 μm length of the apical, middle and basal parts of the OC explants counted in (1) controls (n = 6), (2) gentamicin-treated (500 μM) samples (GM, n = 15), (3) 4 h of GA (2 μM) pretreatment prior to 24 h of gentamicin (500 μM) treatment (GA-pre + GM, n = 8), and (4) concurrent treatment with GA (2 μM) and gentamicin (500 μM) for 24 h (GA + GM parallel, n = 9). Shown are the means ± SEM. B) Geldanamycin protects OHC in apical and middle OC parts from the gentamicin-induced ototoxicity. Number of OHCs per 100 μm length of the apical, middle and basal parts of the OC explants counted in: (1) controls (n = 6), (2) gentamicin-treated (500 μM) samples (GM, n = 15), (3) 4 h of GA (2 μM) pretreatment prior to 24 h of gentamicin (500 μM) treatment (GA-pre + GM, n = 8), and (4) concurrent treatment with GA (2 μM) and gentamicin (500 μM) for 24 h (GA + GM parallel, n = 9). Shown are the means ± SEM. */**/***p < 0.05/0.01/0.001 vs. gentamicin-treated samples.