Figure 10

Real-time RT-PCR of myofibril genes on total RNA from stage 35-37 embryonic hearts treated by MIR. Relative expression levels of different genes were compared after normalizing by beta-actin mRNA levels. 36A: antisense MIR treated for 36 hours; 36S: sense MIR treated for 36 hours; 72A: antisense MIR treated for 72 hours; 72S: sense MIR treated for 72 hours; -/-: homozygous mutant hearts with no treatment; +/+: homozygous normal hearts with no treatment. Alpha-MHC (A), C-protein (B), cardiac troponin T (C), alpha-1-syntrophin (D), p53 (E), Msx-1 (F), beta-1-syntrophin (G), ATmC-3 (H), cardiac actin (I).