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Figure 2 | Journal of Biomedical Science

Figure 2

From: Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts

Figure 2

Confocal microscopy of embryonic axolotl hearts stained with anti-tropomyosin antibody. Normal heart incubated with Steinberg's solution and lipofectin only has extensive tropomyosin staining (A). Mutant heart incubated with Steinberg's solution and lipofectin only has virtually no staining (B). Mutant heart incubated with Steinberg's solution, lipofectin and full length sense MIR shows significant tropomyosin (arrow) staining (C). Mutant heart incubated with Steinberg's solution, lipofectin and full length sense MIR pretreated with RNase. Very little tropomyosin is indicated in the cells (D). Mutant heart incubated with Steinberg's solution, lipofectin and full length antisense MIR. No staining for tropomyosin is observed (E). Mutant heart incubated with Steinberg's solution, lipofectin and 166 nt MIR RNA shows significant levels of tropomyosin (arrow) protein (F); Magnification: 60 ×.

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Journal of Biomedical Science

ISSN: 1423-0127