Figure 4

Transcriptional levels of various tropomyosin isoforms (A1 and A2); Determination of mRNA splicing of various tropomyosin isoforms (B1, B2 and B3). Real-time RT-PCR showed no significant difference at the transcription level for alpha- (A1) and TM4 type tropomyosin (A2) genes in the mutant hearts treated with MIR sense or antisense RNA. 36A: Mutant hearts treated with antisense MIR for 36 hours; 36S: Mutant hearts treated with sense MIR for 36 hours; 72A: Mutant hearts treated with antisense MIR for 72 hours; 72S: Mutant hearts treated with sense MIR for 72 hours; +/+: homozygous normal hearts with no treatment; (-/-): homozygous mutant hearts with no treatment. Real-time RT-PCR showing no significant difference during mRNA splicing of the alpha-TM gene (the ATmC-1 containing exon 2b (B1), the ATmC-2 containing exon 2a (B2) and the fibroblast tropomyosin isoform with direct exon 1b and 3 conjunction (B3) in the mutant hearts treated with the MIR sense or MIR antisense.