Table 1 Primers used for different genes in real time RT-PCR experiments.
Gene | Forward/Reverse | Primer Sequence | Product Size |
|---|---|---|---|
ATmC-3 | Forward | 5'-ggagcttgaccatgcgctgaa | 200 |
ATmC-3 | Reverse | 5'-tgagaaccgacacaaagcaagagg | Â |
ATmC-exon2a | Forward | 5'-gcgtgacagggtgctggatgaac (P5) | 154 |
ATmC-exon2b | Forward | 5'-tgaagggtaccgaggacgagttgga (P3) | Â |
ATmC-exon3 | Reverse | 5'-ccagacgctcctgagcacgatcc (P4) | Â |
ATmC-exon345 | Forward | 5'-gagcgtctggccacagccctaca (P1) | 233 |
ATmC-exon345 | Reverse | 5'-tcttccgcacgctccagatcacc (P2) | Â |
ATm5- | Forward | 5'-gccacacctacctgcgagtgttcc | 249 |
ATm5- | Reverse | 5'-gacgctcctgagcacgatccaac | Â |
MIR | Forward | 5'-ttccacccactcgagcgtcaaca | 158 |
MIR | Reverse | 5'-gctcggatatgcgtgcaaccttga | Â |
cTnT | Forward | 5'-ccaagggcttcaccgggctcaa | 173 |
cTnT | Reverse | 5'-tggcagaggtggaatggatcacagg | Â |
α-syntrophin | Forward | 5'-ggactctccaccgcctccctctc | 208 |
α-syntrophin | Reverse | 5'-ccccgcttcatccttcgctctga |  |
β-syntrophin | Forward | 5'-gacttggcttggtgggctgaacg | 243 |
β-syntrophin | Reverse | 5'-aggccattcagttgcagggtgct |  |
Msx1 | Forward | 5'-tgtacgccgctcacatgggctac | 220 |
Msx1 | Reverse | 5'-ccctcttcctgccaccgaatcac | Â |
Msx2 | Forward | 5'-ggaagggaagggaaggcagaacg | 215 |
Msx2 | Reverse | 5'-tccacgaatcagggcgttgtttg | Â |
β-actin | Forward | 5'-tccatgaaggctgcccaact | 210 |
β-actin | Reverse | 5'-tggcgccacatctgattgat |  |