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Table 1 Primers used for PCR.

From: Amino acid residues 201-205 in C-terminal acidic tail region plays a crucial role in antibacterial activity of HMGB1

  Forward primer Reverse primer
rHMGB1 5'-CTGGTACCATGG 5'-CGCAAGCTTTTATGTC
A box GCAAAGGAGATC-3' TCCCCTTTGGGAGG-3'
rHMGB1 5'-CTGGTACCATGA 5'-CGCAAGCTTTTAATA
B box AGTTCAAGGATCCC-3' TGCAGCAATATCCT-3'
rHMGB1 5'-CTGGTACCATGG 5'-CGCAAGCTTTTATT
  GCAAAGGAGATC-3' CATCATCATCATC-3'
tHMGB1 5'-CTGGTACCATGG 5'-CGCAAGCTTTTACTT
  GCAAAGGAGATC-3' CTTTTTCTTGCT-3'
mHMGB1 5'-CTGGTACCATGG 5'-CGCAAGCTTTTATTC
-211~215 GCAAAGGAGATC-3' TTCTTCATCTTC-3'
mHMGB1 5'-CTGGTACCATGG 5'-CGCAAGCTTTTAATCTT
-206~215 GCAAAGGAGATC-3' CTTCATCTTCC-3'
mHMGB1 5'-CTGGTACCATGG 5'-CGAAGCTTTTACTC
-201~215 GCAAAGGAGATC-3' CTCCTCCTCATCCT-3'
mHMGB1 5'-CTGGTACCATGG 5'-GCAAGCTTTTACTCTT
-196~215 GCAAAGGAGATC-3' CATCTTCCTCATC-3'
mHMGB1 5'-CTGGTACCATGG 5'-CGCAAGCTTTTAATC
-191~215 GCAAAGGAGATC-3' TTCCTCCTCTTCC-3'
mHMGB1 5'-GAAGATGAAGAA 5'-CTTCTTTTTCTT
-186~200 GATGAAGATGAA-3' GCTTTTTTCAGC-3'
mHMGB1 5'-GATGATGATG 5'-CTCTTCATCTT
-196~210 ATGAATAAAAG-3' CCTCATCTT-3'
mHMGB1 5'-GAAGATGAAGA 5'-CTCTTCATCTT
-196-205 AGAAGATGATG-3' CCTCATCTT-3'
mHMGB1 5'-GAAGAAGAT 5'-CTCATCCTCTTC
-198~207 GATGATGATG-3' ATCTTCCTCAT-3'
mHMGB1 5'-GATGATGATGAT 5'-CTCCTCCTCCT
-201-210 GAATAAAAGC-3' CATCCTCTT-3'
mHMGB1 5'-GAAGATGAAGA 5'-CTCCTCCTCCT
-201~205 AGAAGATGATG-3' CATCCTCTT-3'