Table 1 Primers used for PCR.
| Â | Forward primer | Reverse primer |
|---|---|---|
rHMGB1 | 5'-CTGGTACCATGG | 5'-CGCAAGCTTTTATGTC |
A box | GCAAAGGAGATC-3' | TCCCCTTTGGGAGG-3' |
rHMGB1 | 5'-CTGGTACCATGA | 5'-CGCAAGCTTTTAATA |
B box | AGTTCAAGGATCCC-3' | TGCAGCAATATCCT-3' |
rHMGB1 | 5'-CTGGTACCATGG | 5'-CGCAAGCTTTTATT |
| Â | GCAAAGGAGATC-3' | CATCATCATCATC-3' |
tHMGB1 | 5'-CTGGTACCATGG | 5'-CGCAAGCTTTTACTT |
| Â | GCAAAGGAGATC-3' | CTTTTTCTTGCT-3' |
mHMGB1 | 5'-CTGGTACCATGG | 5'-CGCAAGCTTTTATTC |
-211~215 | GCAAAGGAGATC-3' | TTCTTCATCTTC-3' |
mHMGB1 | 5'-CTGGTACCATGG | 5'-CGCAAGCTTTTAATCTT |
-206~215 | GCAAAGGAGATC-3' | CTTCATCTTCC-3' |
mHMGB1 | 5'-CTGGTACCATGG | 5'-CGAAGCTTTTACTC |
-201~215 | GCAAAGGAGATC-3' | CTCCTCCTCATCCT-3' |
mHMGB1 | 5'-CTGGTACCATGG | 5'-GCAAGCTTTTACTCTT |
-196~215 | GCAAAGGAGATC-3' | CATCTTCCTCATC-3' |
mHMGB1 | 5'-CTGGTACCATGG | 5'-CGCAAGCTTTTAATC |
-191~215 | GCAAAGGAGATC-3' | TTCCTCCTCTTCC-3' |
mHMGB1 | 5'-GAAGATGAAGAA | 5'-CTTCTTTTTCTT |
-186~200 | GATGAAGATGAA-3' | GCTTTTTTCAGC-3' |
mHMGB1 | 5'-GATGATGATG | 5'-CTCTTCATCTT |
-196~210 | ATGAATAAAAG-3' | CCTCATCTT-3' |
mHMGB1 | 5'-GAAGATGAAGA | 5'-CTCTTCATCTT |
-196-205 | AGAAGATGATG-3' | CCTCATCTT-3' |
mHMGB1 | 5'-GAAGAAGAT | 5'-CTCATCCTCTTC |
-198~207 | GATGATGATG-3' | ATCTTCCTCAT-3' |
mHMGB1 | 5'-GATGATGATGAT | 5'-CTCCTCCTCCT |
-201-210 | GAATAAAAGC-3' | CATCCTCTT-3' |
mHMGB1 | 5'-GAAGATGAAGA | 5'-CTCCTCCTCCT |
-201~205 | AGAAGATGATG-3' | CATCCTCTT-3' |