Figure 4

Effects of HG/SMG on the adipogenic differentiation of rBMSCs. rBMSCs were cultured under HG/SMG conditions with or without adipogenic inducement medium for 1, 3, 5 or 7 days of a total culture period of 14 days. RT-PCR analysis of PPARγ2 mRNA expression. (A) Electrophoresis graph of PCR products, (B) Gray intensity analysis of electrophoresis bands. Cont, cells were cultured under normal condition without inducer; A, rBMSCs were cultured with inducer for adipocyte differentiation; H, HG culture; M, SMG culture; the numbers 1, 3, 5, 7 indicate the days of HG/SMG culture. Take samples: AH7, cells were cultured under HG conditions for 7 days with adipogenic inducer; M3, cells were cultured under SMG conditions for 3 days without inducer. SMG-/HG-, SMG/HG treated without inducer; SMG+/HG+, SMG/HG treated with inducer. * noted p < 0.05 and ** P < 0.01 compared with relative 0 d group (Cont, A) using student t-test analysis (n = 3). The experiments were conducted twice. The GAPDH house-keeping gene was used as a control. (C) Oil red-O staining to detect the adipogenic differentiation of rBMSCs under HG and SMG conditions. a) The control group. b) The AH₇ showed few oil droplets. c) The AM₇ group contained oil droplets in the cells. Magnification × 200. SMG conditions increased the expression of PPARγ2.