Figure 5
From: Gravity, a regulation factor in the differentiation of rat bone marrow mesenchymal stem cells
Effects of HG on rBMSCs differentiation into force-sensitive cells. rBMSCs were cultured under HG conditions for 7 or 21 days. Cells were then analyzed by FACS with a cTnT-specific monoclonal antibody to detect cardiomyogenic differentiation, or a Cbfa1-specific antibody to detect osteoblastic differentiation. C, static control group; H, HG group. HG increased the numbers of Cbfa1- and cTnT-positive cells compared with the control group, particularly after HG culture for 21 days.