Table 1 Rat primers used for RT-PCR analysis.
From: Gravity, a regulation factor in the differentiation of rat bone marrow mesenchymal stem cells
Gene | Â | Primer sequence | Length (bp) |
|---|---|---|---|
GATA4 | Forward | 5'CTGTCATCTCACTATGGGCA | 310 bp |
| Â | Reverse | 5'CCAAGTCCGAGCAGGAATTT | Â |
β-MHC | Forward | 5'TGGCACCGTGGACTACAATA | 100 bp |
| Â | Reverse | 5'TACAGGTGCATCAGCTCCAG | Â |
ColIA1 | Forward | 5'GTGGATGGCTGCACGAGTC | 243 bp |
| Â | Reverse | 5'TGAGTTTGGGTTGTTGGTCTGT | Â |
Cbfa1 | Forward | 5'CACGACAACCGCACCATG | 165 bp |
| Â | Reverse | 5'GTCCCATCTGGTACCTCTCCG | Â |
ALP | Forward | 5'GGAAGGGTCAGTCAGGTT | 366 bp |
| Â | Reverse | 5'GTGGGCCGCTCTAGGCACCAA | Â |
PPARγ2 | Forward | 5' TTGATTTCTCCAGCATTTC | 360 bp |
| Â | Reverse | 5' GCTCTACTTTGATCGCACT | Â |
GAPDH | Forward | 5'ACCACAGTCCATGCCATCAC | 452 bp |
| Â | Reverse | 5'TCCACCACCCTGTTGCTGTA. | Â |