Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform

Table 1 List of genes and RT-PCR primers used

Gene	Sequence	Annealing Temperature (°C)	Product Size(bp)
Oct 4	CGACCATCTGCCGCTTTGAG CCCCCTGTCCCCCATTCCTA	57	572
Nanog	CCTCCTCCATGGATCTGCTTATTCA CAGGTCTTCACCTGTTTGTAG	57	262
GAPDH	GGGCGCCTGGTCACCAGGGCTG GGGGCCATCCACAGTCTTCTG	60	531
βIIITubulin	CTTGGGGCCCTGGGCCTCCGA GGCTTCCTGCAGTGGTACACGGGCG	60	174
GATA4	TCCAAACCAGAAAACGGAAG CTGTGCCCGTAGTGAGATGA	60	187
Brachury	ACCCAGTTCATAGCGGTGAC ATGAGGATTTGCAGGTGGAC	60	216
BMP2	TGTATCGCAGGCACTCAGGTCAG AAGTCTGGTCACGGGGAAT	60	328
GATA2	TGACTTCTCCTGCATGCACT AGCCGGCACCTGTTGTGCAA	60	244
HAND1	TGCCTCAGAAAGAGAACCAG ATGGCAGGATGAACAAACAC	60	274
BMP4	GTCCTGCTAGGAGGCGCGAG GTTCTCCAGATGTTCTTTCG	60	339
βhCG	GCTACTGCCCCACCATGACC ATGGACTCGAAGCGCACATC	55	95
GCM1	CGGAAACGCTGTCCCAACT CCCTTCAGGCTCAATGAGACG	57	235
IGF2	CAATGGGGAAGTCGATGCTG CTTGGCGAGCACGTGAC	61	421
FGFR1	GGACTCTCCCATCACTCTGCAT CCCCTGTGCAATAGATGATGATC	56	109

ISSN: 1423-0127