Figure 1
Effect of puerarin in middle cerebral artery occlusion (MCAO)-induced focal cerebral ischemia in rats. (A) Coronal sections of TTC-stained brains, (B) dose-response effect of puerarin, and (C) infarct area at various distances from the frontal pole in 24 h after MACO-reperfusion rats. Cerebral infarction in sham-operated (sham) or MACO-reperfusion rats is from a representative animal that received solvent (solvent; cremophor: ethanol: normal saline at 1: 1: 4) or puerarin (25 and 50 mg/kg) intraperitoneally. (B) Infarct volumes were calculated as described in "Methods", and data are presented as a superimposed scatterplot showing the infarct volume for each animal in the group as well as the means ± S.E.M. (n = 5). ***P < 0.001 compared to the sham-operated group; ###P < 0.001 compared to the solvent-treated group. (C) Forebrain profiles of the infarct area at various distances from the frontal pole as described in "Methods". Each point (○, solvent-treated group; △, puerarin 50 mg/kg-treated group) and vertical bar represent the means ± S.E.M. (n = 5). *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the solvent-treated group.