Figure 1

Measurement of adhesin mRNA expression and invertible element orientation in UPEC J96 grown under different conditions. (A) Analysis of adhesins FimH and PapG mRNA expression in J96 grown in either pH 7.0 with no NaCl medium or pH 5.5 with 400 mM NaCl medium. RNA samples from J96 were isolated and subjected to RT-PCR analysis. (B) The PCR was performed with chromosomal DNA isolated from J96 grown in pH 7.0 with no NaCl condition or in pH 5.5 with 400 mM NaCl condition, using the INV and FIMA primers to amplify phase-on-oriented DNA, and the FIME and INV primers to amplify phase-off-oriented DNA. The dilutions were used for PCR as follows: undiluted (lanes 1), 1/2 (lanes 2), 1/4 (lanes 3), 1/8 (lanes 4), 1/16 (lanes 5), 1/32 (lanes 6). All PCR products were separated by agarose gel electrophoresis and stained with ethidium bromide.