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Figure 3 | Journal of Biomedical Science

Figure 3

From: Difference in the regulation of IL-8 expression induced by uropathogenic E. coli between two kinds of urinary tract epithelial cells

Figure 3

Induction of IL-8 expression in uroepithelail cells by different fimbriated J96 invasion. RNA samples were isolated at the indicated time periods with the infection with indicated fimbrial types, followed by subjecting to real-time PCR analysis. Data are normalized against 18S rRNA level and presented as fold changes in fluorescent density in comparison to that of control ECs (CL) (A-D). The IL-8 protein secretion in conditioned media was determined by ELISA analyses (E,F). 5637 or 786-O cells were kept as controls (CL) or invasion with either type 1 or P fimbriated J96 for the times indicated (A,B), or the cells were invaded with different fimbrial types of J96 for 4 h (C,E) or 12 h (D,F). Data are shown as mean ± standard error of the mean (SEM). * P < 0.05 versus control epithelial cells (CL).

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