Figure 4

Induction of IL-8 expression in uroepithelail cells by non-fimbriated E. coli 83972 and P-fimbriated 83972 (83972-P) invasion. (A) 5637 or 786-O cells were kept as controls (CL) or invasion with non-fimbriated 83972 or 83972-P for 4 h, RNA samples were isolated and subjected to real-time PCR analysis. Data are normalized against 18S rRNA level and presented as fold changes in fluorescent density in comparison to that of control ECs (CL). (B) The IL-8 protein secretion in conditioned media was determined by ELISA analyses. 5637 or 786-O cells were kept as controls (CL) or invasion with non-fimbriated 83972 or 83972-P for 12 h. Data are shown as mean ± standard error of the mean (SEM). * P < 0.05 versus control epithelial cells (CL).