Figure 6

Effect of MAPK inhibitors on the regulation of IL-8 expression in uroepithelail cells. (A) 5637 cells were kept as controls (CL) or invaded with type 1 fimbriated J96 (J96-1) for 4 h. (B) 786-O cells were kept as controls (CL) or invaded with P fimbriated J96 (J96-P) for 4 h. Before being kept as controls or invaded with J96, cells were pretreated with PD98059 (PD), SP600125 (SP), or SB203580 (SB) separately for 1 h. Data are normalized against 18S rRNA level and presented as fold changes in comparison to control cells (CL) and. The results are shown as mean ± SEM. * P < 0.05 versus CL. ^#P < 0.05 versus DMSO-treated cells with J96 invasion. ^&P < 0.01 versus DMSO-treated cells with J96 invasion. (C) and (D) The phosphorylation of p38 in 5637 cells (C) and 786-O cells (D) after 30 min of either J96-1 or J96-P invasion was determined by using Western blot.