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Figure 7 | Journal of Biomedical Science

Figure 7

From: Difference in the regulation of IL-8 expression induced by uropathogenic E. coli between two kinds of urinary tract epithelial cells

Figure 7

J96 invasion induced p65 NF-κB and AP-1 binding activities. (A) NF-κB and AP-1 activation by type 1 fimbriated J96 (J96-1) invasion in 5637 cells, and (B) NF-κB and AP-1 activation by P fimbriated J96 (J96-P) invasion in 786-O cells were determined by TF ELISA assay. All bar graphs represent folds of control cells (CL), mean ± SEM. * P < 0.05 versus p65 NF-κB activation in CL. #P < 0.05 versus AP-1 activation in CL. (C) 5637 cells were kept as controls (CL) or invaded with J96-1 for 4 h. (D) 786-O cells were kept as controls (CL) or invaded with J96-P for 4 h. Before being kept as controls or invaded with J96, cells were pretreated with NF-κB inhibitor Pyrrolidine dithiocarbamate (PDTC), or AP-1 inhibitor Tanshinone IIA (TIIA) individually for 1 h. Data are normalized to 18S rRNA level and presented as fold changes in comparison to control cells (CL) and. The results are shown as mean ± SEM. * P < 0.05 versus CL. #P < 0.05 versus DMSO-treated cells with J96 invasion. &P < 0.01 versus DMSO-treated cells with J96 invasion.

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