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Figure 1 | Journal of Biomedical Science

Figure 1

From: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells

Figure 1

Procedures of clone screening after RHKD. RHKD tet-inducible lentiviral vector design (top) and screening procedure of 3T3-L1 cells for adipogenesis with RHKD. CMV/TRE, cytomegalovirus/tetracycline response element; GFP, green fluorescent protein; PGK, phosphoglycerate kinase promoter; Blast, blasticidin-S deaminase for selection against blasticidin after lentiviral infection; LTR, long terminal repeat. Left microphotograph shows a clone of 3T3-L1 cells (GFP-positive) after RHKD, which have little lipid accumulation inside the cells, surrounded by non-infected (GFP-negative) and properly differentiated 3T3-L1 adipocytes. Right microphotograph shows a clone of 3T3-L1 cells (GFP-positive) after RHKD, which have over accumulated lipid inside the cells, compared to surrounding non-infected (GFP-negative) and properly differentiated 3T3-L1 adipocytes.

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