Figure 1

Induction of PAR-1, PAR-2, PAR-3, and PAR-4 mRNA level by IL-1β in h PSCs and chondrocytes and Induction of PAR-2 mRNA and protein level by IL-1β in h PSCs. (A). h PSCs and chondrocytes were incubated in 10 ng/ml of IL-1β in serum-free DMEM for 30, 60, and 90 minutes following 24 hours of starvation and PAR-1, PAR-2, PAR-3, and PAR-4 mRNA level of h PSCs and chondrocytes were determined by RT-PCR (n = 3). Lane 1 as basal control and lane 2 to lane 4 were compared with basal control. (B). h PSCs were incubated in 10 ng/ml of IL-1β in serum-free DMEM for 30, 60, and 90 minutes following 24 hours of starvation (n = 3). h PSCs were incubated with 10 ng/ml of IL-1β for 12, 24, and 48 hours (n = 3). Lane 1 as basal control and lane 2 to lane 4 were compared with basal control.