Figure 7

TIMP-3 blocks MMP-13 activity and blocking of MMP-13 function results in down-regulation of PAR-2 protein level of h PSCs. (A) h PSCs were incubated with 10 ng/ml of IL-1β in serum-free DMEM for 12 hours and then co-incubated with 10 ng/ml of TGF-β1 in serum-free DMEM for 12 hours (n = 3). Lane 1 as basal control and lane 2 as positive control, lane 3 was compared with positive control. (B) h PSCs were incubated with 2, 1, 0.5, and 0.1 ng/ml of MMP-13 in serum-free DMEM for 24 hours (n = 3). Lane 1 as basal control and lane 2 to lane 5 were compared with basal control.