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Figure 2 | Journal of Biomedical Science

Figure 2

From: The specificity of neuroprotection by antioxidants

Figure 2

Assessing Cellular Levels of Superoxide with the Superoxide Indicator MitoSOX Red. HT-22 cells were stained with 5 μM MitoSOX red for 10 min at 37°C before imaging. A: Control. B: The cells were treated with 10 μg/ml oligomycin for 2 hr. C: The cells were treated with 10 μg/ml oligomycin and 50 μM FeTCPP for 2 hr. D: The cells were treated with 1 μM myxothiazol for 2 hr. E: The cells were treated with 10 μg/ml oligomycin for 2 hr and stained with MitoSOX red that had been oxidized by superoxide from the xanthine/xanthine oxidase system. F: The cells were treated with 10 μg/ml oligomycin for 2 hr and stained with MitoSOX red that went through the same xanthine/xanthine oxidase procedure as in E except that xanthine oxidase was omitted to prevent the formation of superoxide.

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