Figure 5

Effects of TNFα on Mitochondrial Membrane Potential, Cellular ROS Levels and Cell Viability in L929 Cells. A: Control. B: The cells were treated with 10 ng/ml TNFα and 1 μg/ml actinomycin D for 16 hr. C: The cells were treated with 10 ng/ml TNFα and 1 μg/ml actinomycin D plus 100 μM amytal for 16 hr. D: The cells were treated with 10 ng/ml TNFα and 1 μg/ml actinomycin D for the indicated periods of time. E: The cells were treated with 10 ng/ml TNFα and 1 μg/ml actinomycin D alone or plus 100 μM of the indicated agents for 3 hr. F: The cells were treated with 10 ng/ml TNFα and 1 μg/ml actinomycin D alone or plus 100 μM of the indicated agents for 16 hr. *: Significantly different from TNFα-treated cells by paired Student's t-test (p < 0.05).