Cyclic stretch increases toll-like receptor 4 (TLR4) protein and mRNA expression in cardiomyocytes. (A) Representative Western blots for TLR4 in cardiomyocytes subjected to cyclic stretch by 20% or 10% for various periods of time. (B) Quantitative analysis of TLR4 protein levels. The values from stretched cardiomyocytes have been normalized to values in control cells and the data are from 4 independent experiments. *P < 0.001 vs. control. **P < 0.01 vs. control. (n = 4 per group) (C) Fold increases in TLR4 mRNA as a result of cyclic stretch by 20% for various periods of time. The values from stretched cardiomyocytes have been normalized to matched GAPDH measurement and then expressed as a ratio of normalized values to mRNA in control cells (n = 4 per group). *P < 0.01 vs. control.