Cyclic stretch increases release of TNF-α and angiotensin II (AngII) from cardiomyocytes subjected to 20% of stretch for various periods of time. The cultured medium were collected for measurement of TNF-α (A) and Ang II (B) in cultured cardiomyocytes after stretch for various periods of time via immunoassay (n = 4). *P < 0.001 vs. control. **P < 0.05 vs. control. +P < 0.001 vs. stretch 2 h.