Table 1 Primers used for semi-quantitative RT-PCR
Gene | Forward primer | Reverse primer |
|---|---|---|
Ect2 | 5'-GAGAGCTGGCTGAAGATGCT-3' | 5'-TCCTCTGAGCTATGGGATG-3' |
Cadm1 | 5'-TCTGGGCCAAACCTGTTCATC AAT-3' | 5'-CTGTGTCTGCGTCTGCTGCGTCAT-3' |
Rgs4 | 5'-GTTGCTCCCGCTGCCTTTCTCT-3' | 5'-CATGTTCCGGCTTGTCTCCTCTC-3' |
Cldnd1 | 5'-AACTTGGGATTGTGGAGACG-3' | 5'-CAGAGGTAGGTCCGAAGCAG-3' |
Kntc2 | 5'-CCAGTTAGCTGTGCAGACCA-3' | 5'-CTTCAGACGTGCATTCCTCA-3' |
Socs5 | 5'-GTGGACATGAACGCCAACAG-3' | 5'-AATCTCTGCGGCACAGTTTTG-3' |
Pbrm1-predicted | 5'-TGCCCTTGTCCTCCATAAAG-3' | 5'-AAAGTTCGGATCCACAGCAG-3' |
Apool | 5'-TGAAAAATGGGGTCATGGAT-3' | 5'-CTAATGTGGCCAGTCCCAGT-3' |
MafK | 5'-CCCAAGCCCAACAAGACATT-3' | 5'-GCTGCTTCAGCCGAGTTACC-3' |
SytI | 5'-CCGGACTGACTGATGGAGAAG-3' | 5'-ATCGGATGTACCCCCCATGT-3' |
Syn-1 | 5'-CTCAAACTGCCTCTTCTGCTAATC-3' | 5'-ACTGTCCGTTCAAAGGGTCTATC-3' |
Gapdh | 5'-GGTTACCAGGGCTGCCTTCT-3' | 5'-ATGGGTTTCCCGTTGATGAC-3' |