Skip to main content
Figure 2 | Journal of Biomedical Science

Figure 2

From: Homo-binding character of LMO2 isoforms and their both synergic and antagonistic functions in regulating hematopoietic-related target genes

Figure 2

MBP-pulldown and mammalian two hybrid assay indicated homo-interaction character of LMO2-L and -S isoform. (A) Coomassie blue R-250 staining of MBP-pulldown samples after SDS-PAGE. NC, amylose resin without recombinant protein and incubated with K562/Jurkat total protein; MAL-β-galactase, sample of purified MAL-β-galactase incubated with K562/Jurkat total protein used as a control; MAL-LMO2-L/-S, sample of purified MAL-LMO2-L/-S incubated with K562/Jurkat total protein. (B) Western blot analysis of relative samples corresponding to (A) using anti-LMO2 antibody. (C) Mammalian two hybrid assay of LMO2-L and LMO2-S. pACT, activation domain fusion protein expression plasmid; pBIND, GAL4 binding domain fusion protein expression plasmid. +, cells transfected with such component; -, cells transfected without such component.

Back to article page