Figure 4

Regulation activities of LMO2 isoforms on five element arrangements within a typical LMO2 binding site. (A) Sequences of promoter regions of GPA, VE-Cadherin and miR-142. Letters bigger and in red were indicated as E-box, and in blue as GATA site. (B) Sketch map of the reporter construction strategies. The two black boxes as well as their commentaries indicated 5 different arrangement forms of LMO2 binding site. (C) The artificial sequences used were shown in the table. Letters bigger and in red were indicated as E-box, and in blue as GATA site. (D) Relative activity of each artificial reporter in K562 cells. (E) Relative activity of each artificial reporter in HEK293 cells. For both (D) and (E), the activity of pGL3-promoter was used as control and marked as 1. All these experiments were performed at least 5 times independently, the graph showed the means and the standard error of means of each result. All the data were evaluated by mean square test and Dunnett-t test, *Dunnett-t test, p < 0.05.