Figure 6

Differential regulation of LMO2-L and -S isoforms on endogenous expression of their target genes in K562 cells. (A) Transfection efficiency was monitored by the observation of EGFP fluorescence and estimated more than 80%. (B) Total expression levels of LMO2-L or LMO2-S in cells transfected with EGFP, LMO2-L or LMO2-S. (C) Relative expression levels of miR-142, GPA and c-kit in the same sample of (B). The bars in (B) and (C) represented the ratio between the expression levels of LMO2-L/S and housekeeping gene GAPDH. The expression levels in the cells transfected with EGFP were considered having no prominent changes and used as control. The data showed the means of three independent experiments. All the data were evaluated by student's t-test, *student's t-test, p < 0.05.