Western blot analysis of α-galactosidase A expression in liver and kidney at 6, 12, and 24 weeks after treatment in Fabry mice. Liver and kidney tissue lysates were immunoblotted using anti-α-galactosidase A antibodies. α-Gal A protein expression in liver and kidney at 6, 12, and 24 weeks after injection is demonstrated in (A), and levels of α-Gal A in liver (B) and kidney (C) were quantified using a bioimaging analyzer. Experiments were repeated approximately three to five times using each sample. Values are expressed as means ± SD (n = 3 or 4). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. GAPDH using Student's t test. Black bar: 6 weeks after injection (n = 5), gray bar: 12 weeks after injection (n = 3), white bar: 24 weeks after injection (n = 3).