Skip to main content

Table 1 α-Gal A enzyme activity in the tissues of mice after tail vein administration of rAAV2/8-hAGA vector

From: Characterization of Fabry mice treated with recombinant adeno-associated virus 2/8-mediated gene transfer

Mice group Weeks after injection Enzyme activity (nmol/h/mg protein)
   Liver Kidney Spleen Heart Brain
Wild-type mice   75.7 ± 29.3 63.6 ± 20.5 189.7 ± 23.2 55.1 ± 7.18 111.8 ± 12.2
Fabry mice   1.2 ± 0.13 1.3 ± 0.47 4.3 ± 0.97 0.3 ± 0.16 1.4 ± 0.26
Treated mice
2 × 1011 a
6 1861.4 ± 45.2** 423.2 ± 24.5*** 2036.1 ± 47.0** 1837.2 ± 40.1*** 106.9 ± 8.1
  12 186.1 ± 65.7* 161.7 ± 62.1* 1059.9 ± 423.7** 1263.0 ± 152.0** 66.3 ± 3.2
  24 90.8 ± 42.7 19.4 ± 0.5 305.9 ± 14.1 31.4 ± 4.04* 10.0 ± 2.1
Treated mice
2 × 1012 b
6 2137.5 ± 80.9*** 1267.6 ± 30.8*** 6413.8 ± 336.9*** 4614.7 ± 179.8*** 310.3 ± 7.5***
  12 1062.3 ± 189.8*** 600.6 ± 392.2*** 4276.1 ± 214.1*** 1297.9 ± 746.0*** 263.0 ± 87.2*
  24 734.4 ± 79.2*** 270.1 ± 4.5** 1216.3 ± 44.8*** 601.6 ± 13.2*** 257.0 ± 18.6*
  48 366.2 ± 22.8*** 127.0 ± 14.7** 451.0 ± 9.7** 81.1 ± 10.3** 247.2 ± 30.3*
  60 245.5 ± 95.0*** 64.0 ± 23.7 335.7 ± 11.8** 23.2 ± 1.3 37.7 ± 9.2
Treated mice
ERTc
6 84.3 ± 15.4* 30.7 ± 7.5 96.7 ± 26.5 67.18 ± 4.6* 142.4 ± 37.6**
  1. Data are presented as average ± SD, amice treated with 2 × 1011: rAAV2/8-hAGA (2 × 1011 particles/mouse). b mice treated with 2 × 1012: rAAV2/8-hAGA (2 × 1012 particles/mouse), c ERT treated mice: 1.0 mg/kg once a week for 6 consecutive weeks. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. wild mice (paired t test), n = 3.