Protection against UV toxicity by DDB2 in Drosophila. (A) Expression of hDDB2-GFP in Drosophila. Protein extracts from GFP and GFP-hDDB2 expressing embryos were immunoblotted with anti-GFP antibodies. (B) Protection effect of hDDB2 against UV irradiation in fly larvae. GFP or hDDB2 overexpressing larvae were collected and then irradiated with UV (0-100 J/m2). Four days later, surviving adults were counted and the survival rate was calculated. (n>4000). (C) Lack of inhibition of Reaper- or Eiger-induced apoptosis by hDDB2 over-expression. Eye morphology of wild-type and Reaper/eiger-overexpressing flies were examined. To analyze the effect of hDDB2, GMRGal4 was used to drive the expression of Reaper (GMR>Rpr) or Eiger (GMR>eiger) simultaneously with hDDB2 (GMR>Rpr, UAS-hDDB2 and GMR>eiger, UAS-hDDB2, respectively). Panel a-d: Reaper-induced apoptosis, resulting in small eyes, was not rescued by DDB2 overexpression. Panel a, Eye morphology of wild-type OreR fly; Panel b, Eye morphology of Rpr overexpressing fly; Panel c, Eye morphology of Rpr and DDB2 overexpressing fly; Panel d, Eye morphology of Rpr and P35 overexpressing fly. Panel e~g: Eiger-induced apoptosis not rescued by DDB2 overexpression. Panel e, Eye morphology of wild-type OreR fly; Panel f, Eye morphology of eiger overexpressing fly; Panel g, Eye morphology of eiger and DDB2 overexpressing fly.