Figure 1

Differentiated podocytes express decreased levels of ADAM10 and L1 adhesion molecule protein on the cell surface. Flow cytometry histograms represents number of podocytes (cell counts, y axis) and the fluorescence intensity (x axis) of ADAM10 (A) and L1 adhesion molecule (B) and the isotype-matched control IgG antibody (filled peak) in undifferentiated (green peak) and 9 days differentiated cells (red peak). (C) Western Blot analysis from lysates of undifferentiated podocytes (HPC undiff.), 4 days differentiated podocytes (HPC 4 d diff.) and 9 days differentiated podocytes ((HPC 9 d diff.) with an ADAM10 specific antibody. Blots were stripped and re-probed with an antibody specific for β-actin as a loading control. (D) Western blot analysis of the supernatants of undifferentiated (HPC undiff.) and 9 days differentiated podocytes (HPC 9 d diff.) with L1-11A, an antibody specific for the ectodomain of L1 adhesion molecule. (E) Cell lysates were analyzed by western blot technique with a L1 specific antibody (pcyt). β-actin western blot was used as a loading control.