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Table 1 Primers and probes for real-time PCR.

From: Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin inhibits the proliferation of ARPE-19 cells

Gene ID Gene name Sequence* Amplicon size(bp)
NM_000925 PDHB F: 5'-GAAACCATAGAAGCCAGTGTCA-3'
R: 5'-TCTTTGCATAAGGCATAGGGA-3'
P: 5'-TGTGGAAGGAGGCTGGCCACA-3'
181
NM_005809 PRDX2 F: 5'-AGGTGAAGCTGTCGGACTACA-3'
R: 5'-TGCTGAACGCGATGATCTC-3'
P:5'-CGTGGTCCTCTTTTTCTACCCTCTGGA-3'
99
NM_006406 PRDX4 F: 5'-CACTTCTACGCGGGTGGA-3'
R: 5'-CGCTGGCTTGGAAATCTT-3'
P: 5'-CGCCGACCACTCCCTGCACC-3'
102
NM_005345 HSPA1A F: 5'-CTGCGACAGTCCACTACCTTTT-3'
R: 5'-TCCCTGCTCTCTGTCGGC-3'
P: 5'-CCAAGGCTTCCCAGAGCGAAC-3'
187
NM_002156 HSPD1 F: 5'-GGAGTGGCTGTGCTGAAGGT-3'
R: 5'-GCATCGAAGGAGGGCACA-3'
P: 5'-CTGCTGTTGAAGAAGGCATTGTTTTG-3'
144
NM_001017963 HSP90AA1 F: 5'-TTCAGACAGAGCCAAGGTGC-3'
R: 5'-CAATGACATCAACTGGGCAAT-3'
P: 5'-CCCAGACCCAAGACCAACCGATGG-3'
168
NM_007355 HSP90AB1 F: 5'-GGCAGTCAAGCACTTTTCTGTAG-3'
R: 5'-GTCAACCACACCACGGATAAA-3'
P: 5'-ATTGCTATTTATTCCTCGTCGGGCT-3'
199
NM_002046 GAPDH F: 5-TGCACCACCAACTGCTTAGC-3'
R: 5'-TCTTCTGGGTGGCAGTGATG-3'
P: 5'-ATGGACTGTGGTCATGAGTCCTTCCA-3'
106
NM_001100 β-actin F: 5'-GGCACCCAGCACAATGAA-3'
R: 5'-GGAAGGTGGACAGCGAGG-3'
P: 5'-CAAGATCATTGCTCCTCCTGAGCGC-3'
98
  1. *F: forward; R: reverse; P: probe