Figure 2From: The signals of FGFs on the neurogenesis of embryonic stem cellsThe FGF effects on the neurogenesis of ES cells and the FGFR expressions in ES cells. (A) After treatment with FGF1, FGF2, FGF4, and FGF8b from day 1 to day 3 using the SFEB method, the numbers of 46C ES-derived Sox1-GFP+ cells were estimated by flow cytometry on day 6 (n = 3 for each panel). (B) On indicated days, FGFRs in 46C ES cells were analyzed by RT-PCR. (C) Expression of FGFRs and the GFP+ ES cells was analyzed by immunostaining on day 6 or day 2. Single GFP positive cells were indicated by arrow. Nuclei of all cells are revealed by DAPI staining in blue. Scale bar, 10 μm in C. *, p < 0.01, Anova test.Back to article page