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Figure 2 | Journal of Biomedical Science

Figure 2

From: PKCα mediated induction of miR-101 in human hepatoma HepG2 cells

Figure 2

Identification of key regulatory miRNA in TPA induced growth arrest in HepG2 cells. (A) Expression levels of 270 miRNAs in 20 pairs of human HCC tissues. The labeled miRNAs were inversely modulated in TPA-treated HepG2 cells and dotted lines indicate the 2-fold change threshold. Expression levels of miRNA were presented as 39-Ct. (B) Expression levels of miR-29c and miR-101 in 20 pair of human HCC tissues and their adjacent normal tissues. Expression levels of miRNA were presented as 39-Ct. p-values were calculated using T-test. (C) Time-dependent changes in miR-101, miR-29c and miR-122 expression levels. HepG2 cells were treated with 100 nM TPA for indicated time periods and the total RNAs were collected for stem-loop RT-qPCR. Expression levels of miR-101, miR-29c and miR-122 were normalized to miR-16 and expressed as fold-change using time 0 as baseline.

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