Figure 1
From: Trypsin-induced proteome alteration during cell subculture in mammalian cells
Cell dissociation workflow with and without trypsin digestion. (A) The dissociation time for attached MCF-7 cells were determined where 90% confluent cells in 96-well cell culture plates were gently washed with HBSS twice followed by treated with 100 μl of non-enzymatic cell dissociation solution or 0.05% trypsin-EDTA. After indicated treatment times, cells were gently washed with HBSS and the number of adherent cells counted. The mean cell number of 4 independent assays is shown +/- SD. (B) Overview of cell dissociation workflow with and without trypsin digestion of adhesive MCF-7 cells.