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Table 1 PCR Primers used to amplify promoter region and exons 18, 19, 20, and 21 of the EGFR genes

From: Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer

EGFR gene Sequence
E18-5' 5'-CTGGCACTGCTTTCCAGCAT-3'
E18-3' 5'-GCTTGCAAGGACTCTGGGCT-3'
E19-5' 5'-GCATCGCTGGTAACATCCAC-3'
E19-3' 5'-AGATGAGCAGGGTCTAGAGC-3'
E20-5' 5'-ATCGCATTCATGCGTCTTCA-3'
E20-3' 5'-AGACCGCATGTGAGGATCCT-3'
E21-5' 5'-TGACCCTGAATTCGGATGCA-3'
E21-3' 5'-ATACAGCTAGTGGGAAGGCA-3'
Promoter 5' 5'-CCTCCTCTGCTCCTCCCGAT-3'
Promoter 3' 5'-CGGGGCTAGCTCGGGACT-3'