Table 2 Primer extension mutation analysis probes for -216 promoter region and codons 719, 746-750, 790, and 858 of the EGFR genes

2155 G>T, 2155 G>A

G719C, G719S

5'-TGAATTCAAAAAGATCAAAGTGCTG-3'

25 mer

2156 G>C

G719A

5'-AAACTGAATTCAAAAAGATCAAAGTGCTGG-3'

30 mer

2235-2249 del

E746-A750 del

5'-GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAA-3'

35 mer

2236-2250 del

E746-A750 del

5'-TCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAG-3'

41 mer

2237-2254 del

E746-T751 del

5'-(T)20AGTTAAAATTCCCGTCGCTATCAAGG-3'

46 mer

2240-2257 del

L747-S752 del

5'-(T)23AGTTAAAATTCCCGTCGCTATCAAGGAAT-3

52 mer

2573 T>G

L858R

5'-(T)26ACCGCAGCATGTCAAGATCACAGATTTTGGGC-3'

58 mer

2369 C>T

T790M

5'-CTCCACCGTGCAGCTCATCA-3'

20 mer

-216 G/T

5'-GGCCGCAGCAGCCTCC-3'

16 mer