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Table 2 Primer extension mutation analysis probes for -216 promoter region and codons 719, 746-750, 790, and 858 of the EGFR genes

From: Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer

Nucleotide number and sequence Amino acid change Sequence Size
2155 G>T, 2155 G>A G719C, G719S 5'-TGAATTCAAAAAGATCAAAGTGCTG-3' 25 mer
2156 G>C G719A 5'-AAACTGAATTCAAAAAGATCAAAGTGCTGG-3' 30 mer
2235-2249 del E746-A750 del 5'-GAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAA-3' 35 mer
2236-2250 del E746-A750 del 5'-TCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAG-3' 41 mer
2237-2254 del E746-T751 del 5'-(T)20AGTTAAAATTCCCGTCGCTATCAAGG-3' 46 mer
2240-2257 del L747-S752 del 5'-(T)23AGTTAAAATTCCCGTCGCTATCAAGGAAT-3 52 mer
2573 T>G L858R 5'-(T)26ACCGCAGCATGTCAAGATCACAGATTTTGGGC-3' 58 mer
2369 C>T T790M 5'-CTCCACCGTGCAGCTCATCA-3' 20 mer
-216 G/T   5'-GGCCGCAGCAGCCTCC-3' 16 mer