Figure 5

Observation of subcellular localization using confocal laser scanning microscopy. The subcellular location of wild type and mutant prM was visualized by double-staining of the infected Sf9 cells. The ER compartment was stained with 10 nM DiIC13(3), and the prM proteins were stained with mAb 5B1, followed by goat anti-mouse Alexa Fluor 647 IgG. Photographs were taken with appropriate excitation laser wavelengths and merged to reveal the co-localization of prM and ER compartment.