Figure 6

Sucrose gradient sedimentation analysis of alanine-insertion mutagenesis of the anchor region of prM protein. Sf9 cells were coinfected with Bac-E and Bac-prM mutants. Cell lysates labeled with [³⁵S] were applied for centrifugation in a 3 to 60% (wt/wt) sucrose gradient. Each fraction was immunoprecipitated with monoclonal antibody E3.3. The immune complexes were analyzed by reducing SDS-PAGE and fluorography. (A) Sf9 cells coinfected with Bac-prM and Bac-E; (B) Sf9 cells coinfected with prM A139 and Bac-E; (C) Sf9 cells coinfected with prM A143 and Bac-E; (D) Sf9 cells coinfected with prM A147 and Bac-E; (E) Sf9 cells coinfected with prM A157 and Bac-E; (E) Sf9 cells coinfected with prM A157 and Bac-E; (F) Sf9 cells coinfected with prM A161 and Bac-E; (G) Sf9 cells coinfected with prM A165 and Bac-E. The data presented in this figure are three independent experiments.